The machine has three lasers with emission wavelengths of 635 (red), 532 (green) and 473 (blue) nm, and we have filters available for a wide range of fluores including Cy2 and C圓. Fuji FLA-5100 Phosphoimager is a large format high-resolution laser scanner designed for filmless autoradiography and imaging of gels and membranes dyed with fluorescent stains.GE/Amersham Imager 600RGB is a sensitive and robust CCD-based imager for use with chemiluminescence, UV and white light transillumination, and multifluorescence analysis of protein and DNA samples in gels and membranes.Detailed information concerning the capabilities and uses of these instruments and their analysis software can be found HERE (Pitt username required). These machines are respectively housed in 572 and 563A Crawford Hall, and are available for use by members of the Biology Department. The Department of Biological Sciences maintains several versatile state-of-the-art digital imaging systems as well as a multimodal microplate reader that combine high sensitivity with the ability to perform rigorous quantitation and analysis of a wide range of experimental applications.
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